Pair Name	Shikonin, Anti-mouse PD-1
Disease Info	[ICD-11: 2B91.Z]	Colorectal cancer		Investigative
Gene Regulation	Down-regulation	Expression	CALR	hsa811
	Down-regulation	Expression	HMGB1	hsa3146
	Up-regulation	Expression	HSPA4	hsa3308
In Vitro Model	CT26	Mouse colon adenocarcinoma	Mus musculus (Mouse)	CVCL_7254
In Vivo Model	6×10⁵ CT26 cells were subcutaneously inoculated into BALB/c mice in the right inguinal region.
Result	Our study elucidated the potential role of 'Shikonin-PKM2-ROS-Hsp70' axis in the promotion of efficacy of PD-1 blockade in CRC treatments, providing a potential strategy and targets for improving the efficacy of PD-1 blockade in colorectal cancer.